德州驴NCAPG-DCAF16基因区域多态性与生长性状的关联分析

doi:10.11843/j.issn.0366-6964.2019.02.008

摘要/Abstract

摘要：

旨在探讨NCAPG-DCAF16基因区域在德州驴群体中的遗传多态性以及与生长性状的关联性，进而得到显著相关的遗传标记，为德州驴的分子标记辅助选择提供理论基础。本研究采用DNA混池测序技术对NCAPG-DCAF16基因区域的多态性进行检测；基于DNA混池测序结果，运用质谱分型技术对227头德州驴群体中的SNPs位点进行基因型检测，分析基因型与初生、3月龄、6月龄3个时期相关生长性状的关联性。结果表明：1）通过DNA混池测序发现，NCAPG基因上的SNPs位点包括第9内含子上rs1（T>C），第11内含子上rs2（A>G），第19内含子上rs4（C>G），第8内含子上rs5（C>G）；DCAF16基因第2外显子发现一个同义突变，即位点rs008（A>G）。2）初生时期的关联分析显示，rs1位点基因型CC个体的平均尻长显著高于CT型个体（P<0.05）；3月龄时期各基因型的表型平均值无显著差异（P>0.05）；在6月龄阶段，基因型为TT个体的平均胸宽与CC个体相比差异极显著（P<0.01），TT和CT基因型个体的平均管围均显著高于CC个体（P<0.05）。3）rs4位点的关联分析揭示了GG基因型个体在初生时期的平均胸深显著高于GC基因型（P<0.05），3月龄时期各基因型之间的平均表型值无显著差异（P>0.05）；CC基因型个体6月龄时期的平均胸宽和管围显著高于GG基因型（P<0.05）。4）rs008位点关联分析结果表明，各基因型之间初生时期的表型值无显著差异（P>0.05）；AA、GA基因型个体3月龄时期的体重、体高和尻高都显著高于GG基因型（P<0.05）；AA、GA基因型个体6月龄时期的体高和胸围都极显著高于GG基因型个体（P<0.01），GA基因型与GG基因型个体间的胸深、尻高的差异达到极显著水平（P<0.01），GA基因型个体的尻宽显著高于GG和AA基因型（P<0.05）。5）根据连锁不平衡分析结果，rs1、rs2、rs4和rs5的组合基因型在德州驴群体中有5种。关联分析结果揭示了TTAACCCC组合基因型个体6月龄时期的平均胸宽显著高于CCGGGGGG组合基因型（P<0.05）。综上，德州驴群体中NCAPG-DCAF16基因区域5个SNPs位点对生长性状均有显著影响，特别是rs008位点与德州驴体重、体高等重要生长性状极显著相关，可用于德州驴的分子标记辅助选择，提高德州驴育种效率。

Abstract:

The study aimed to investigate the genetic polymorphisms of NCAPG-DCAF16 region in Dezhou donkeys and their associations with growth traits, which can help to mine significant genetic markers for molecular marker assisted selection of Dezhou donkeys. The polymorphisms of NCAPG-DCAF16 region in Dezhou donkeys were detected by DNA pool sequencing technology. Based on the results of DNA pool sequencing, mass-array system was used to genotype the detected SNPs in 227 Dezhou donkeys. Association analysis between the SNPs of NCAPG-DCAF16 region and the growth traits at birth, 3 months old and 6 months old were performed. The results showed that:1) Four SNPs were detected in NCAPG gene, rs1 (T>C) in the 9th intron, rs2 (A>G) in the 11th intron, rs4 (C>G) in the 19th intron, and rs5 (C>G) in the 8th intron. A synonymous mutation was found in the 2nd exon of DCAF16 gene, and the site was rs008 (A>G). 2) The correlation analysis at birth revealed that the average rump length of the individuals with CC genotype at the rs1 was significantly higher than those with CT genotype at birth (P<0.05), the phenotypic values of individuals with different genotypes at 3 months old had no significant difference (P>0.05). At 6 months old, the average chest width of individuals with TT genotype was extremely significantly different from those with CC genotype (P<0.01), and the average shin circumference of individuals with TT and CT genotypes was significantly higher than those with CC genotype (P<0.05). 3) The correlation analysis of rs4 locus indicated that the average chest depth of individuals with GG genotype was significantly higher than those with GC genotype at birth (P<0.05). At 3 months old, the average phenotypic values of individuals with different genotypes was not significantly different (P>0.05). The average chest width and shin circumference of individuals with CC genotype at the age of 6 months were significantly higher than those with GG genotype (P<0.05). 4) The results of rs008 locus correlation analysis showed that there was no significant difference in phenotypic values among individuals with different genotypes at birth (P>0.05). The body weight, body height and rump height of individuals with AA and GA genotypes were significantly higher than those with GG genotypes at the age of 3 months (P<0.05). At 6 months old, the body height and chest circumference of individuals with AA and GA genotypes were extremely significantly higher than those with GG genotype (P<0.01). The difference in chest depth and rump height between individuals with GA and GG genotypes was extremely significantly different (P<0.01). The rump width of individuals with GA genotype was significantly higher than those with GG and AA genotypes (P<0.05). 5) According to the result of linkage disequilibrium analysis, rs1, rs2, rs4 and rs5 had 5 combination genotypes in Dezhou donkeys. The results of association analysis displayed that the average chest width of individuals with TTAACCCC genotype was significantly higher than those with CCGGGGGG genotype at 6 months old (P<0.05). In summary, the 5 SNPs in the NCAPG-DCAF16 region of the Dezhou population have the significant effects on growth traits, especially the rs008 locus has a significant correlation with the important growth traits such as body weight and body height, which can be used as genetic markers to improve the breeding efficiency in Dezhou donkeys.

中图分类号:

S822.2

侯浩宾, 李海静, 杨莉, 张新浩, 石田培, 王欣悦, 赵志达, 张莉. 德州驴NCAPG-DCAF16基因区域多态性与生长性状的关联分析[J]. 畜牧兽医学报, 2019, 50(2): 302-313.

HOU Haobin, LI Haijing, YANG Li, ZHANG Xinhao, SHI Tianpei, WANG Xinyue, ZHAO Zhida, ZHANG Li. Association between NCAPG-DCAF16 Region Polymorphisms and Growth Traits in Dezhou Donkeys[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2019, 50(2): 302-313.

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